Candida albicans Shed Msb2 and Host Mucins Affect the Candidacidal Activity of Salivary Hst 5

Pathogens

Sumant Puri²  Justin Friedman²  Darpan Saraswat²  Rohitashw Kumar²  Rui Li²  Donna Ruszaj¹  Mira Edgerton² 

[1] Department of Pharmaceutical Sciences, University at Buffalo, Buffalo, NY 14214, USA; E-Mail:;Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, NY 14214, USA; E-Mails:

关键词: Candida albicans;    oral candidiasis;    Histatin 5;    saliva;    mucins;    secreted aspartyl proteases;   

DOI  :  10.3390/pathogens4040752

来源: mdpi

【 摘 要 】

Salivary Histatin 5 (Hst 5) is an antimicrobial peptide that exhibits potent antifungal activity towards Candida albicans, the causative agent of oral candidiasis. However, it exhibits limited activity in vivo, largely due to inactivation by salivary components of both host and pathogen origin. Proteins secreted by C. albicans during infection such as secreted aspartyl proteases (Saps) and shed mucin Msb2 can reduce Hst 5 activity; and human salivary mucins, while suggested to protect Hst 5 from proteolytic degradation, can entrap peptides into mucin gels, thereby reducing bioavailability. We show here that Sap6 that is secreted during hyphal growth reduces Hst 5 activity, most likely a result of proteolytic degradation of Hst 5 since this effect is abrogated with heat inactivated Sap 6. We further show that just like C. albicans shedding Msb2, mammalian mucins, fetuin and porcine gut mucin (that is related to salivary mucins), also reduce Hst 5 activity. However, we identify mucin-like protein-induced changes in C. albicans cell morphology and aggregation patterns, suggesting that the effect of such proteins on Hst 5 cannot be interpreted independently of their effect on yeast cells.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.