Chemotaxonomic Metabolite Profiling of 62 Indigenous Plant Species and Its Correlation with Bioactivities

Molecules

Sarah Lee¹  Dong-Gu Oh²  Sunmin Lee²  Ga Ryun Kim¹  Jong Seok Lee¹  Youn Kyoung Son¹  Chang-Hwan Bae¹  Joohong Yeo¹  Choong Hwan Lee² 

[1] National Institute of Biological Resources, Environmental Research Complex, Incheon 404-708, Korea;Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea;

关键词: chemotaxonomy;    indigenous plant;    metabolite profiling;    UHPLC-LTQ-IT-MS/MS;    antioxidant activity;    tyrosinase inhibition activity;   

DOI  :  10.3390/molecules201119652

来源: mdpi

【 摘 要 】

Chemotaxonomic metabolite profiling of 62 indigenous Korean plant species was performed by ultrahigh performance liquid chromatography (UHPLC)-linear trap quadrupole-ion trap (LTQ-IT) mass spectrometry/mass spectrometry (MS/MS) combined with multivariate statistical analysis. In partial least squares discriminant analysis (PLS-DA), the 62 species clustered depending on their phylogenetic family, in particular, Aceraceae, Betulaceae, and Fagaceae were distinguished from Rosaceae, Fabaceae, and Asteraceae. Quinic acid, gallic acid, quercetin, quercetin derivatives, kaempferol, and kaempferol derivatives were identified as family-specific metabolites, and were found in relatively high concentrations in Aceraceae, Betulaceae, and Fagaceae. Fagaceae and Asteraceae were selected based on results of PLS-DA and bioactivities to determine the correlation between metabolic differences among plant families and bioactivities. Quinic acid, quercetin, kaempferol, quercetin derivatives, and kaempferol derivatives were found in higher concentrations in Fagaceae than in Asteraceae, and were positively correlated with antioxidant and tyrosinase inhibition activities. These results suggest that metabolite profiling was a useful tool for finding the different metabolic states of each plant family and understanding the correlation between metabolites and bioactivities in accordance with plant family.

【 授权许可】

CC BY   
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