Heat Killed Lactobacillus reuteri GMNL-263 Reduces Fibrosis Effects on the Liver and Heart in High Fat Diet-Hamsters via TGF-β Suppression

International Journal of Molecular Sciences

Wei-Jen Ting⁸  Wei-Wen Kuo⁵  Dennis Jine-Yuan Hsieh¹  Yu-Lan Yeh⁶  Cecilia-Hsuan Day²  Ya-Hui Chen³  Ray-Jade Chen⁴  Viswanadha Vijaya Padma⁷  Yi-Hsing Chen³  Chih-Yang Huang⁸ 

[1] School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40201, Taiwan; E-Mail:;Department of Nursing, Mei Ho University, Pingtung 91201, Taiwan; E-Mail:;Research and Development Department, GenMont Biotech Incorporation, Tainan 74144, Taiwan; E-Mail:;Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan; E-Mail:;Department of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan; E-Mail:;Department of Pathology, Changhua Christian Hospital, Changhua 50006, Taiwan; E-Mail:;Department of Biotechnology, Bharathiar University, Coimbatore 641046, India; E-Mail:;Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan; E-Mail:

关键词: Lactobacillus reuteri GMNL-263;    transforming growth factor β;    cardiovascular diseases;   

DOI  :  10.3390/ijms161025881

来源: mdpi

【 摘 要 】

Obesity is one of the major risk factors for nonalcoholic fatty liver disease (NAFLD), and NAFLD is highly associated with an increased risk of cardiovascular disease (CVD). Scholars have suggested that certain probiotics may significantly impact cardiovascular health, particularly certain Lactobacillus species, such as Lactobacillus reuteri GMNL-263 (Lr263) probiotics, which have been shown to reduce obesity and arteriosclerosis in vivo. In the present study, we examined the potential of heat-killed bacteria to attenuate high fat diet (HFD)-induced hepatic and cardiac damages and the possible underlying mechanism of the positive effects of heat-killed Lr263 oral supplements. Heat-killed Lr263 treatments (625 and 3125 mg/kg-hamster/day) were provided as a daily supplement by oral gavage to HFD-fed hamsters for eight weeks. The results show that heat-killed Lr263 treatments reduce fatty liver syndrome. Moreover, heat-killed Lactobacillus reuteri GMNL-263 supplementation in HFD hamsters also reduced fibrosis in the liver and heart by reducing transforming growth factor β (TGF-β) expression levels. In conclusion, heat-killed Lr263 can reduce lipid metabolic stress in HFD hamsters and decrease the risk of fatty liver and cardiovascular disease.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.