Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates

Biosensors

Peter Owens²  Nigel Phillipson¹  Jayakumar Perumal³  Gerard M. O𠆜onnor¹  Malini Olivo¹ 

[1] School of Physics, National University Ireland, University Road, Galway, Ireland; E-Mails:;Centre for Microscopy and Imaging, National University Ireland, University Road, Galway, Ireland;Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios 138667, Singapore; E-Mails:

关键词: Raman spectroscopy;    protein sensing;    surface-enhanced Raman scattering;    biomarker;   

DOI  :  10.3390/bios5040664

来源: mdpi

【 摘 要 】

In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein–antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.