Marine Drugs | |
Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes | |
Pascal Viens2  Marie-Ève Lacombe-Harvey2  Ryszard Brzezinski1  | |
[1] Département de Biologie, Faculté des Sciences, Université de Sherbrooke, 2500, boul. de l’Université, Sherbrooke, QC J1K 2R1, Canada; | |
关键词:
chitosan;
chitosanase;
glycoside hydrolase;
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DOI : 10.3390/md13116566 | |
来源: mdpi | |
【 摘 要 】
Chitosanases, enzymes that catalyze the endo-hydrolysis of glycolytic links in chitosan, are the subject of numerous studies as biotechnological tools to generate low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS) from native, high molecular weight chitosan. Glycoside hydrolases belonging to family GH46 are among the best-studied chitosanases, with four crystallography-derived structures available and more than forty enzymes studied at the biochemical level. They were also subjected to numerous site-directed mutagenesis studies, unraveling the molecular mechanisms of hydrolysis. This review is focused on the taxonomic distribution of GH46 proteins, their multi-modular character, the structure-function relationships and their biological functions in the host organisms.
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190004138ZK.pdf | 2141KB | download |