Structure of an Amino Acid-Decorated Exopolysaccharide Secreted by a Vibrio alginolyticus Strain

Marine Drugs

Sophie Drouillard²  Isabelle Jeacomine²  Laurine Buon²  Claire Boisset²  Anthony Courtois¹  Bertrand Thollas¹  Pierre-Yves Morvan³  Romuald Vallée³  William Helbert² 

[1] Polymaris Biotechnology, Aéropôle Centre, 29600 Morlaix, France; E-Mails:;Université Grenoble Alpes, CERMAV, CNRS, 38000 Grenoble, France; E-Mails:;Codif International, 35610 Roz-sur-Couesnon, France; E-Mails:

关键词: exopolysaccharide;    Vibrio alginolyticus;    amino acids;    ingredient;   

DOI  :  10.3390/md13116723

来源: mdpi

【 摘 要 】

Vibrio alginolyticus (CNCM I-4994) secretes an exopolysaccharide that can be used as an ingredient in cosmetic applications. The structure was resolved using chromatography and one- and two-dimensional NMR spectroscopy experiments. The results show that the carbohydrate backbone is made of two residues: d-galacturonic acid and N-acetyl-d-glucosamine (GlcNac), which together constitute a tetrasaccharide repetition unit: [→3)-α-d-GalA-(1→4)-α-d-GalA-(1→3)-α-d-GalA-(1→3)-β-GlcNAc(1→]. Two amino acids, alanine and serine, are linked to GalA residues via amido linkages. The position and the distribution of the amino acids were characterized by two-dimensional NMR spectroscopy. To our knowledge, this is the first description of a structure for a marine exopolysaccharide decorated with an amino acid.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.