Proteomics Analysis of Cellular Proteins Co-Immunoprecipitated with Nucleoprotein of Influenza A Virus (H7N9)

International Journal of Molecular Sciences

Ningning Sun²  Wanchun Sun³  Shuiming Li¹  Jingbo Yang²  Longfei Yang²  Guihua Quan²  Xiang Gao²  Zijian Wang²  Xin Cheng²  Zehui Li²  Qisheng Peng³  Ning Liu² 

[1] College of Life Sciences, Shenzhen University, Shenzhen 518057, China;Central Laboratory, Jilin University Second Hospital, Changchun 130041, China;Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China;

关键词: nucleoprotein;    influenza A virus;    lysine acetylation;    mass spectrometry;    co-immunoprecipitation;   

DOI  :  10.3390/ijms161125934

来源: mdpi

【 摘 要 】

Avian influenza A viruses are serious veterinary pathogens that normally circulate among avian populations, causing substantial economic impacts. Some strains of avian influenza A viruses, such as H5N1, H9N2, and recently reported H7N9, have been occasionally found to adapt to humans from other species. In order to replicate efficiently in the new host, influenza viruses have to interact with a variety of host factors. In the present study, H7N9 nucleoprotein was transfected into human HEK293T cells, followed by immunoprecipitated and analyzed by proteomics approaches. A series of host proteins co-immunoprecipitated were identified with high confidence, some of which were found to be acetylated at their lysine residues. Bioinformatics analysis revealed that spliceosome might be the most relevant pathway involved in host response to nucleoprotein expression, increasing our emerging knowledge of host proteins that might be involved in influenza virus replication activities.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.