Radical Scavenging by Acetone: A New Perspective to Understand Laccase/ABTS Inactivation and to Recover Redox Mediator

Molecules

Hao Liu²  Pandeng Zhou¹  Xing Wu²  Jianliang Sun²  Shicheng Chen³ 

[1]School of Chemistry and Environmental Engineering, Hunan City University, Yiyang 413000, China

[2]State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China

[3]

[4]Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA

关键词: laccase;    ABTS;    inhibition;    radical scavenging;    recovery;   

DOI  :  10.3390/molecules201119672

来源: mdpi

【 摘 要 】

The biosynthetic utilization of laccase/mediator system is problematic because the use of organic cosolvent causes significant inhibition of laccase activity. This work explored how the organic cosolvent impacts on the laccase catalytic capacity towards 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) in aqueous solution. Effects of acetone on the kinetic constants of laccase were determined and the results showed K_m and V_max varied exponentially with increasing acetone content. Acetone as well as some other cosolvents could transform ABTS radicals into its reductive form. The content of acetone in media significantly affected the radical scavenging rates. Up to 95% of the oxidized ABTS was successfully recovered in 80% (v/v) acetone in 60 min. This allows ABTS to be recycled at least six times with 70%–75% of active radicals recovered after each cycle. This solvent-based recovery strategy may help improve the economic feasibility of laccase/ABTS system in biosynthesis.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.