Global Profiling of Various Metabolites in Platycodon grandiflorum by UPLC-QTOF/MS

International Journal of Molecular Sciences

Jae Won Lee²  Seung-Heon Ji²  Geum-Soog Kim²  Kyung-Sik Song³  Yurry Um²  Ok Tae Kim²  Yi Lee⁴  Chang Pyo Hong¹  Dong-Ho Shin⁵  Chang-Kug Kim⁵  Seung-Eun Lee²  Young-Sup Ahn²  Dae-Young Lee² 

[1] Theragen Etex, Suwon 443-270, Korea;Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea;College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea;Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 361-763, Korea;Genomics Division, National Academy of Agricultural Science (NAAS), Jeonju 560-500, Korea;

关键词: Platycodon grandiflorum;    metabolomics;    UPLC-QTOF/MS;   

DOI  :  10.3390/ijms161125993

来源: mdpi

【 摘 要 】

In this study, a method of metabolite profiling based on UPLC-QTOF/MS was developed to analyze Platycodon grandiflorum. In the optimal UPLC, various metabolites, including major platycosides, were separated well in 15 min. The metabolite extraction protocols were also optimized by selecting a solvent for use in the study, the ratio of solvent to sample and sonication time. This method was used to profile two different parts of P. grandiflorum, i.e., the roots of P. grandiflorum (PR) and the stems and leaves of P. grandiflorum (PS), in the positive and negative ion modes. As a result, PR and PS showed qualitatively and quantitatively different metabolite profiles. Furthermore, their metabolite compositions differed according to individual plant samples. These results indicate that the UPLC-QTOF/MS-based profiling method is a good tool to analyze various metabolites in P. grandiflorum. This metabolomics approach can also be applied to evaluate the overall quality of P. grandiflorum, as well as to discriminate the cultivars for the medicinal plant industry.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.