Derinat Protects Skin against Ultraviolet-B (UVB)-Induced Cellular Damage

Molecules

Wen-Li Hsu³  Jian-He Lu⁶  Mami Noda¹  Ching-Ying Wu⁶  Jia-Dai Liu¹  Manabu Sakakibara⁷  Ming-Hsien Tsai⁴  Hsin-Su Yu⁵  Ming-Wei Lin²  Yaw-Bin Huang²  Shian-Jang Yan³  Tohru Yoshioka⁴ 

[1] Laboratory of Pathophysiology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8581, Japan;School of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan;The Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan;Lipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung 80708, Taiwan;Department of Dermatology, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan;Graduate Institute of Medicine, School of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan;School of High-Technology for Human Welfare, Tokai University, 410-0321 Numazu, Shizuoka, Japan;

关键词: Derinat;    UVB;    ROS;    calcium;    TRPCs;   

DOI  :  10.3390/molecules201119693

来源: mdpi

【 摘 要 】

Ultraviolet-B (UVB) is one of the most cytotoxic and mutagenic stresses that contribute to skin damage and aging through increasing intracellular Ca²⁺ and reactive oxygen species (ROS). Derinat (sodium deoxyribonucleate) has been utilized as an immunomodulator for the treatment of ROS-associated diseases in clinics. However, the molecular mechanism by which Derinat protects skin cells from UVB-induced damage is poorly understood. Here, we show that Derinat significantly attenuated UVB-induced intracellular ROS production and decreased DNA damage in primary skin cells. Furthermore, Derinat reduced intracellular ROS, cyclooxygenase-2 (COX-2) expression and DNA damage in the skin of the BALB/c-nu mice exposed to UVB for seven days in vivo. Importantly, Derinat blocked the transient receptor potential canonical (TRPC) channels (TRPCs), as demonstrated by calcium imaging. Together, our results indicate that Derinat acts as a TRPCs blocker to reduce intracellular ROS production and DNA damage upon UVB irradiation. This mechanism provides a potential new application of Derinat for the protection against UVB-induced skin damage and aging.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.