The Effect of Trifluoroethanol and Glycerol on the Thermal Properties of Collagen Using Optical Displacement-Enhanced Heterodyne Polarimeter

Applied Sciences

Chien-Ming Wu²  Horn-Haw Chen²  Kai-Han Tseng²  Hung-Wei Chen¹ 

[1] Department of Electronic Engineering, National United University, Miaoli 36063, Taiwan;Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan; E-Mails:

关键词: collagen protection;    heterodyne polarimeter;    thermal denaturation;    trifluoroethanol;    glycerol;   

DOI  :  10.3390/app5041184

来源: mdpi

【 摘 要 】

An angular displacement-enhanced heterodyne polarimeter has been employed to investigate the interplay between trifluoroethanol (TFE) and glycerol on the thermal denaturation of type I collagen. The concentration of the collagen solution was fixed at 0.341 (mg/mL), and was heated from 25 °C to 55 °C. TFE solutions with concentrations of 5%, 10%, 15%, 20%, 40% and 80% (v/v) were prepared and the phase change was recorded for the determination of thermal denaturation. It was observed that the thermal denaturation temperature (T_d) is decreased with increasing TFE concentration due to the partial cleavage of the triple-helical structure. With TFE concentration higher than 20% (v/v), the degree of optical rotation appears to be nearly the same, reflecting that the collagen triple helices have been completely destructed. Moreover, the addition of glycerol in inhibiting the thermal denaturation of collagen is investigated. It has been shown that glycerol can improve the thermal denaturation of both collagen and TFE-mixed collagen. Experimental results show that, in the presence of 2 M glycerol, the T_d of collagen remained at around 41.9 °C, meanwhile the T_d of 20% (v/v) TFE-mixed collagen is significantly restored to 32.8 °C.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.