Metabolic and Hematological Consequences of Dietary Deoxynivalenol Interacting with Systemic Escherichia coli Lipopolysaccharide

Toxins

Erik Bannert¹  Tanja Tesch¹  Jeannette Kluess¹  Jana Frahm¹  Susanne Kersten¹  Stefan Kahlert²  Lydia Renner²  Hermann-Josef Rothkötter²  Sven Dänicke¹ 

[1] Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 50, Braunschweig 38116, Germany; E-Mails:;Institute of Anatomy, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, Magdeburg 39120, Germany; E-Mails:

关键词: swine;    deoxynivalenol;    E. coli lipopolysaccharides;    endotoxin;    sepsis;    blood gas;    metabolism;    glucose;    inflammatory response;   

DOI  :  10.3390/toxins7114773

来源: mdpi

【 摘 要 】

Previous studies have shown that chronic oral deoxynivalenol (DON) exposure modulated Escherichia coli lipopolysaccharide (LPS)-induced systemic inflammation, whereby the liver was suspected to play an important role. Thus, a total of 41 barrows was fed one of two maize-based diets, either a DON-diet (4.59 mg DON/kg feed, n = 19) or a control diet (CON, n = 22). Pigs were equipped with indwelling catheters for pre- or post-hepatic (portal vs. jugular catheter) infusion of either control (0.9% NaCl) or LPS (7.5 µg/kg BW) for 1h and frequent blood sampling. This design yielded six groups: CON_CON_jugular-CON_portal, CON_CON_jugular-LPS_portal, CON_LPS_jugular-CON_portal, DON_CON_jugular-CON_portal, DON_CON_jugular-LPS_portal and DON_LPS_jugular-CON_portal. Blood samples were analyzed for blood gases, electrolytes, glucose, pH, lactate and red hemogram. The red hemogram and electrolytes were not affected by DON and LPS. DON-feeding solely decreased portal glucose uptake (p < 0.05). LPS-decreased partial oxygen pressure (pO₂) overall (p < 0.05), but reduced pCO₂ only in arterial blood, and DON had no effect on either. Irrespective of catheter localization, LPS decreased pH and base-excess (p < 0.01), but increased lactate and anion-gap (p < 0.01), indicating an emerging lactic acidosis. Lactic acidosis was more pronounced in the group DON_LPS_jugular-CON_portal than in CON-fed counterparts (p < 0.05). DON-feeding aggravated the porcine acid-base balance in response to a subsequent immunostimulus dependent on its exposure site (pre- or post-hepatic).

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.