Antiviral Activity of Porcine Interferon Regulatory Factor 1 against Swine Viruses in Cell Culture

Viruses

Yongtao Li²  Hongtao Chang²  Xia Yang²  Yongxiang Zhao²  Lu Chen²  Xinwei Wang²  Hongying Liu²  Chuanqing Wang²  Jun Zhao¹ 

[1] College of Animal Husbandry & Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China

关键词: porcine IRF1;    antiviral activity;    IFN-β;    IFN-stimulated genes;   

DOI  :  10.3390/v7112913

来源: mdpi

【 摘 要 】

Interferon regulatory factor 1 (IRF1), as an important transcription factor, is abundantly induced upon virus infections and participates in host antiviral immune responses. However, the roles of porcine IRF1 (poIRF1) in host antiviral defense remain poorly understood. In this study, we determined that poIRF1 was upregulated upon infection with viruses and distributed in nucleus in porcine PK-15 cells. Subsequently, we tested the antiviral activities of poIRF1 against several swine viruses in cells. Overexpression of poIRF1 can efficiently suppress the replication of viruses, and knockdown of poIRF1 promotes moderately viral replication. Interestingly, overexpression of poIRF1 enhances dsRNA-induced IFN-β and IFN-stimulated response element (ISRE) promoter activation, whereas knockdown of poIRF1 cannot significantly affect the activation of IFN-β promoter induced by RNA viruses. This study suggests that poIRF1 plays a significant role in cellular antiviral response against swine viruses, but might be dispensable for IFN-β induction triggered by RNA viruses in PK-15 cells. Given these results, poIRF1 plays potential roles in cellular antiviral responses against swine viruses.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.