Improved Antioxidant Capacity of Optimization of a Self-Microemulsifying Drug Delivery System for Resveratrol

Molecules

Ying Chen⁴  Huiyong Zhang¹  Jing Yang²  Haiyan Sun²  Thomas Rades³  Holger Grohganz³ 

[1] College of Life Science and Biotechnology, Xinxiang Medical University, Xinxiang 453003, China;School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China;;id="af1-molecules-20-19750">1College of Life Sciences, Henan Normal University, Xinxiang 453007, Chi;College of Life Sciences, Henan Normal University, Xinxiang 453007, China

关键词: resveratrol;    nano-encapsulation;    self-emulsifying;    oil-in-water;    antioxidant;    cytotoxicity;   

DOI  :  10.3390/molecules201219750

来源: mdpi

【 摘 要 】

The use of nano-encapsulated resveratrol (RSV) in self-micro-emulsified drug delivery systems (SMEDDS) formulations was investigated. Self-emulsifying grading tests were used to establish the optimal ratio of oil, surfactant, and co-surfactant. The optimized system was further investigated for the droplet size and zeta potential at the different medium pH values by a Malvern Zetasizer and transmission electron microscopy (TEM). The antioxidant capacity and cytotoxicity of the formulation were detected by DCFH-DA and a CCK-8 assays. The results showed that the nano-emulsion based on ethyl oleate, Tween-80, and PEG-400 (35:40:25, w/w/w) was the most stable formulation due to the small droplet size (approximately 50 nm) and high zeta potential in a neutral environment. Furthermore, this formulation also exhibited a greater antioxidant capacity with less toxicity than free RSV. Taken together, considering these results and the simple fabrication process, this formulation could be used to deliver nutritional food supplements in a stable, efficient, and safe manner.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.