Enrichment and Purification of Polyphenol Extract from Sphallerocarpus gracilis Stems and Leaves and in Vitro Evaluation of DNA Damage-Protective Activity and Inhibitory Effects of α-Amylase and α-Glucosidase

Molecules

Tingting Ma¹  Xiangyu Sun³  Chengrui Tian¹  Jiyang Luo²  Cuiping Zheng¹  Jicheng Zhan³  Isabel C. F. R. Ferreira⁴ 

[1] College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China;National HACCP Center, Chinese Academy of Inspection and Quarantine, Beijing 100123, China;College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;;College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China

关键词: SGslP;    purification;    phenolic composition;    oxidative DNA damage;    enzyme inhibition;   

DOI  :  10.3390/molecules201219780

来源: mdpi

【 摘 要 】

An efficient preparative separation method for Sphallerocarpus gracilis stems and leaves polyphenols (SGslP) was established in this study. An X-5 macroporous adsorption resin was selected for the purification of the SGslP, and the polyphenol content of the purified SGslP (PSGslP) was increased 5.11-fold from 8.29% to 42.38% after one treatment run. The chemical composition of the PSGslP was analyzed by HPLC-MS/MS, and the predominant compounds were found to be luteolin-7-glucoside, acacetin-7-acetyglycoside and its isomers. In addition, the PSGslP was evaluated in vitro to determine the DNA damage-protective activity and inhibitory effects of α-amylase and α-glucosidase. The results indicated that the PSGslP exhibited significant protective activities against both ROO• and •OH radical-induced DNA damage. Moreover, the PSGslP exerted a dose-dependent inhibition effect on α-glucosidase but no inhibitory effect on α-amylase. These findings indicate that the Sphallerocarpus gracilis stems and leaves are good natural sources of antioxidants and are potent inhibitors of α-glucosidase activity and are potential anti-diabetic inhibitor.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.