Alphacoronaviruses Detected in French Bats Are Phylogeographically Linked to Coronaviruses of European Bats

Viruses

Anne Goffard²  Christine Demanche⁵  Laurent Arthur³  Claire Pinçon¹  Johan Michaux⁴  Jean Dubuisson² 

[1] University Lille, CHU Lille, EA 2694-Santé publique: épidémiologie et qualité des soins, F-59000 Lille, France;Molecular & Cellular Virology, University Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 8204-CIIL-Centre d’Infection et d’Immunité de Lille, Bâtiment IBL. 1 rue du Pr. Calmette CS 50447, 59021 Lille Cedex, France;Museum d’Histoire Naturelle de Bourges, Les Rives d’Auron, allée René Ménard, 18000 Bourges, France;Conservation Genetics Unit, Institute of Botany (B. 22), University Liège, 4000 Liège, Belgium;Bacterial Respiratory Infections: Pertussis and Tuberculosis, University Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 8204-CIIL-Centre d’Infection et d’Immunité de Lille, F-59000 Lille, France;

关键词: bats;    alphacoronavirus;    coronavirus;    phylogeographic analysis;    phylogenetic analysis;    Europe;    molecular characterization;   

DOI  :  10.3390/v7122937

来源: mdpi

【 摘 要 】

Bats are a reservoir for a diverse range of viruses, including coronaviruses (CoVs). To determine the presence of CoVs in French bats, fecal samples were collected between July and August of 2014 from four bat species in seven different locations around the city of Bourges in France. We present for the first time the presence of alpha-CoVs in French Pipistrellus pipistrellus bat species with an estimated prevalence of 4.2%. Based on the analysis of a fragment of the RNA-dependent RNA polymerase (RdRp) gene, phylogenetic analyses show that alpha-CoVs sequences detected in French bats are closely related to other European bat alpha-CoVs. Phylogeographic analyses of RdRp sequences show that several CoVs strains circulate in European bats: (i) old strains detected that have probably diverged a long time ago and are detected in different bat subspecies; (ii) strains detected in Myotis and Pipistrellus bat species that have more recently diverged. Our findings support previous observations describing the complexity of the detected CoVs in bats worldwide.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.