Sensors | |
Modulation of Intracellular Quantum Dot to Fluorescent Protein Förster Resonance Energy Transfer via Customized Ligands and Spatial Control of Donor–Acceptor Assembly | |
Lauren D. Field2  Scott A. Walper2  Kimihiro Susumu3  Eunkeu Oh1  Igor L. Medintz2  James B. Delehanty2  | |
[1] Optical Sciences Division, Code 5600 U.S. Naval Research Laboratory, Washington, DC 20375, USA;Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, 4555 Overlook Ave, S.W., Washington, DC 20375, USA;Sotera Defense Solutions, Inc., 7230 Lee DeForest Drive, Columbia, MD 21046, USA; | |
关键词: fluorescence; sensor; FRET; quantum dot; protein; membrane; assembly; noncovalent; ligand; | |
DOI : 10.3390/s151229810 | |
来源: mdpi | |
【 摘 要 】
Understanding how to controllably modulate the efficiency of energy transfer in Förster resonance energy transfer (FRET)-based assemblies is critical to their implementation as sensing modalities. This is particularly true for sensing assemblies that are to be used as the basis for real time intracellular sensing of intracellular processes and events. We use a quantum dot (QD) donor -mCherry acceptor platform that is engineered to self-assemble
【 授权许可】
CC BY
© 2015 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
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RO202003190002135ZK.pdf | 1707KB | download |