Deficiency of iPLA2β Primes Immune Cells for Proinflammation: Potential Involvement in Age-Related Mesenteric Lymph Node Lymphoma

Cancers

Johannes Inhoffen²  Sabine Tuma-Kellner²  Beate Straub¹  Wolfgang Stremmel²  Walee Chamulitrat² 

[1] Pathology Institute of Medical Faculty Heidelberg, 69120 Heidelberg, Germany;Department of Internal Medicine IV, University Heidelberg Hospital, 69120 Heidelberg, Germany;

关键词: Kupffer cells;    lymphocytes;    immune response;    PLA2G6;    CD95/FasL;    M1 and Th1 cytokines;    mesenteric lymph node lymphoma;   

DOI  :  10.3390/cancers7040901

来源: mdpi

【 摘 要 】

Proinflammation can predispose the body to autoimmunity and cancer. We have reported that iPLA₂β^−/− mice are susceptible to autoimmune hepatitis and colitis. Here we determined whether cytokine release by immune cells could be affected by iPLA₂β deficiency alone or combined with CD95/FasL-antibody treatment in vivo. We also determined whether cancer risk could be increased in aged mutant mice. Immune cells were isolated from 3-month old male WT and iPLA₂β^−/− mice, and some were injected with anti-CD95/FasL antibody for 6 h. Kupffer cells (KC) or splenocytes and liver lymphocytes were stimulated in vitro by lipopolysaccharide or concanavalinA, respectively. Whole-body iPLA₂β deficiency caused increased apoptosis in liver, spleen, and mesenteric lymph node (MLN). KC from mutant mice showed suppressed release of TNFα and IL-6, while their splenocytes secreted increased levels of IFNγ and IL-17a. Upon CD95/FasL activation, the mutant KC in turn showed exaggerated cytokine release, this was accompanied by an increased release of IFNγ and IL-17a by liver lymphocytes. Aged iPLA₂β^−/− mice did not show follicular MLN lymphoma commonly seen in aged C57/BL6 mice. Thus, iPLA₂β deficiency renders M1- and Th1/Th17-proinflammation potentially leading to a reduction in age-related MLN lymphoma during aging.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.