Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles

Toxins

Yunxia Luan³  Jiayi Chen³  Cheng Li³  Gang Xie¹  Hailong Fu³  Zhihong Ma³  Anxiang Lu³  Michelangelo Pascale² 

[1] Grain Safety, Academy of State Administration of Grain, Beijing 100037, China;;Agriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaAgriculture Environment, Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;

关键词: aptamer;    Ochratoxin A (OTA);    gold nanoparticles;    cationic polymer;    colorimetric assay;   

DOI  :  10.3390/toxins7124883

来源: mdpi

【 摘 要 】

A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.