Helleborus purpurascens—Amino Acid and Peptide Analysis Linked to the Chemical and Antiproliferative Properties of the Extracted Compounds

Molecules

Adina-Elena Segneanu¹  Ioan Grozescu¹  Florentina Cziple⁴  Daniel Berki²  Daniel Damian¹  Cristina Mariana Niculite³  Alexandru Florea³  Mircea Leabu³ 

[1] National Institute for Research and Development in Electrochemistry and Condensed Matter—INCEMC, Timisoara 300224, Romania;University Politehnica Timisoara, 2 Piata Victoriei, Timisoara 300006, Romania;Department of Morphological Sciences, University of Medicine and Pharmacy “Carol Davila”, 8 Eroilor Sanitari Blvd, Sector 5, Bucharest 050474, Romania;University “Eftimie Murgu”, Resita, 1-4 Traian Vuia, Resita 320085, Romania;

关键词: antiproliferative activiy;    chromatographic techniques;    hellebore;    thionins;    mass spectroscopy;   

DOI  :  10.3390/molecules201219819

来源: mdpi

【 摘 要 】

There is a strong drive worldwide to discover and exploit the therapeutic potential of a large variety of plants. In this work, an alcoholic extract of Helleborus purpurascens (family Ranunculaceae) was investigated for the identification of amino acids and peptides with putative antiproliferative effects. In our work, a separation strategy was developed using solvents of different polarity in order to obtain active compounds. Biochemical components were characterized through spectroscopic (mass spectroscopy) and chromatographic techniques (RP-HPLC and GC-MS). The biological activity of the obtained fractions was investigated in terms of their antiproliferative effects on HeLa cells. Through this study, we report an efficient separation of bioactive compounds (amino acids and peptides) from a plant extract dependent on solvent polarity, affording fractions with unaffected antiproliferative activities. Moreover, the two biologically tested fractions exerted a major antiproliferative effect, thereby suggesting potential anticancer therapeutic activity.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.