Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate

International Journal of Molecular Sciences

Spandan Chaudhary⁴  Surendra K. Chikara⁴  Mahesh C. Sharma¹  Abhinav Chaudhary⁴  Bakhtiyar Alam Syed²  Pooja S. Chaudhary⁴  Aditya Mehta⁴  Maulik Patel⁴  Arpita Ghosh⁴  Marcello Iriti³ 

[1] Department of Biotechnology, Kadi Sarva Vishwavidyalaya, II Floor, KBIPER Building, Kadi Campus, Sector-23, Gandhinagar-382023, Gujarat, India;Department of Biotechnology, Hemchandracharya North Gujarat University, Patan-384265, Gujarat, India;;Department of Agricultural and Environmental Sciences, Milan State University, via G. Celoria 2, Milan 20133, ItalyDepartment of Medical Genetics, Xcelris Labs Limited, Old Premchandnagar Road, Opp. Satyagrah Chhavani, Bodakdev, Ahmedabad-380015, Gujarat, India;

关键词: elicitors;    mevalonate pathway;    steroidal saponins;    3-hydroxy-3-methylglutaryl-CoA reductase;    sterol-3-β-glucosyl transferase;    plant innate immunity;   

DOI  :  10.3390/ijms161226208

来源: mdpi

【 摘 要 】

The effects of methyl jasmonate (MeJA), an elicitor of plant defense mechanisms, on the biosynthesis of diosgenin, a steroidal saponin, were investigated in six fenugreek (Trigonella foenum-graecum) varieties (Gujarat Methi-2, Kasuri-1, Kasuri-2, Pusa Early Branching, Rajasthan Methi and Maharashtra Methi-5). Treatment with 0.01% MeJA increased diosgenin levels, in 12 days old seedlings, from 0.5%–0.9% to 1.1%–1.8%. In addition, MeJA upregulated the expression of two pivotal genes of the mevalonate pathway, the metabolic route leading to diosgenin: 3-hydroxy-3-methylglutaryl-CoA reductase (HMG) and sterol-3-β-glucosyl transferase (STRL). In particular, MeJA increased the expression of HMG and STRL genes by 3.2- and 22.2-fold, respectively, in the Gujarat Methi-2 variety, and by 25.4- and 28.4-fold, respectively, in the Kasuri-2 variety. Therefore, MeJA may be considered a promising elicitor for diosgenin production by fenugreek plants.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.