Tudor-SN Regulates Milk Synthesis and Proliferation of Bovine Mammary Epithelial Cells

International Journal of Molecular Sciences

Jinxia Ao²  Chengjie Wei²  Yu Si²  Chaochao Luo²  Wei Lv²  Ye Lin²  Yingjun Cui²  Xuejun Gao¹ 

[1] The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China

关键词: Tudor-SN;    milk synthesis;    cell proliferation;    Stat5;    NFκB1;   

DOI  :  10.3390/ijms161226212

来源: mdpi

【 摘 要 】

Tudor staphylococcal nuclease (Tudor-SN) is a highly conserved and ubiquitously expressed multifunctional protein, related to multiple and diverse cell type- and species-specific cellular processes. Studies have shown that Tudor-SN is mainly expressed in secretory cells, however knowledge of its role is limited. In our previous work, we found that the protein level of Tudor-SN was upregulated in the nucleus of bovine mammary epithelial cells (BMEC). In this study, we assessed the role of Tudor-SN in milk synthesis and cell proliferation of BMEC. We exploited gene overexpression and silencing methods, and found that Tudor-SN positively regulates milk synthesis and proliferation via Stat5a activation. Both amino acids (methionine) and estrogen triggered NFκB1 to bind to the gene promoters of Tudor-SN and Stat5a, and this enhanced the protein level and nuclear localization of Tudor-SN and p-Stat5a. Taken together, these results suggest the key role of Tudor-SN in the transcriptional regulation of milk synthesis and proliferation of BMEC under the stimulation of amino acids and hormones.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.