Main Leaf Polyphenolic Components of Berry Color Variant Grapevines and Their Acclimative Responses to Sunlight Exposure

Applied Sciences

Marianna Kocsis³  László Abrankó⁴  Ferhan Ayaydin²  Krist༿ Csepregi³  Nóra Papp⁴  Péter Teszlák¹  Gปor Jakab³ 

[1] Research Institute for Viticulture and Oenology, University of Pécs, 4 Pázmány P, Pécs 7632, Hungary; E-Mail:;Cellular Imaging Laboratory, Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, Szeged 6701, Hungary; E-Mail:;Department of Plant Biology, University of Pécs, 6 Ifjúság, Pécs 7624, Hungary; E-Mails:;Department of Applied Chemistry, Corvinus University of Budapest, 29-43 Villányi, Budapest 1118, Hungary; E-Mails:

关键词: grapevine leaves;    polyphenolic profile;    HPLC-ESI-qTOFMS;    fluorescence microimaging;    sunlight acclimation;   

DOI  :  10.3390/app5041955

来源: mdpi

【 摘 要 】

Grapevine leaf synthesizes a wide variety of bioactive secondary metabolites, including polyphenols, which are also key components in ensuring development and growth of the whole plant even under adverse environmental conditions. Our study evaluates the nonanthocyanin polyphenolic composition in grapevine leaves of three varieties of Gohér conculta (Vitis vinifera L.) native to Hungary. A high performance liquid chromatography (HPLC) system including a diode array detector (DAD) coupled to a time-of-flight mass spectrometer (q-TOFMS) was successfully applied to profile intact glycoconjugate forms in samples. In-source fragmentation was utilized in order to provide structural information on the compounds. Using this method, the presence of 16 polyphenolic metabolites were confirmed, and eight of them were subjected to further quantification in sun acclimated and half shaded leaves. Intracellular microimaging detected accumulation of flavonols in cell nuclei, cell wall and chloroplasts. Our findings demonstrated that Gohér conculta—a special grapevine taxon of our viticultural heritage with berry color variants—is a suitable model to study the interaction between genetic and environmental factors in determination of grapevine phenolic composition.

【 授权许可】

CC BY   
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