Flux Balance Analysis Inspired Bioprocess Upgrading for Lycopene Production by a Metabolically Engineered Strain of Yarrowia lipolytica

Metabolites

Komi Nambou¹  Xingxing Jian¹  Xinkai Zhang¹  Liujing Wei¹  Jiajia Lou¹  Catherine Madzak²  Qiang Hua¹  Kazuyuki Shimizu³ 

[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; E-Mails:;INRA, UMR1319 Micalis, Domaine de Vilvert, F-78352 Jouy-en-Josas, France; E-Mail:State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

关键词: Yarrowia lipolytica;    lycopene;    flux balance analysis;    fermentation;    metabolic network model yli v1.7;   

DOI  :  10.3390/metabo5040794

来源: mdpi

【 摘 要 】

Genome-scale metabolic models embody a significant advantage of systems biology since their applications as metabolic flux simulation models enable predictions for the production of industrially-interesting metabolites. The biotechnological production of lycopene from Yarrowia lipolytica is an emerging scope that has not been fully scrutinized, especially for what concerns cultivation conditions of newly generated engineered strains. In this study, by combining flux balance analysis (FBA) and Plackett-Burman design, we screened chemicals for lycopene production from a metabolically engineered strain of Y. lipolytica. Lycopene concentrations of 126 and 242 mg/L were achieved correspondingly from the FBA-independent and the FBA-assisted designed media in fed-batch cultivation mode. Transcriptional studies revealed upregulations of heterologous genes in media designed according to FBA, thus implying the efficiency of model predictions. Our study will potentially support upgraded lycopene and other terpenoids production from existing or prospect bioengineered strains of Y. lipolytica and/or closely related yeast species.

【 授权许可】

CC BY   
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