Detection of Airborne Inoculum of Hymenoscyphus fraxineus and H. albidus during Seasonal Fluctuations Associated with Absence of Apothecia

Forests

Milon Dvorak²  Gabriela Rotkova³  Leticia Botella²  Jan Stenlid¹  Jonas Oliva¹ 

[1] Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech RepublicDepartment of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic;Department of Experimental Biology, Masaryk University, Kamenice 735/5, 625 00 Brno, Czech Republic;

关键词: Chalara fraxinea;    ash dieback;    qPCR detection;    spore trap;    Fraxinus;    leaf wetness;   

DOI  :  10.3390/f7010001

来源: mdpi

【 摘 要 】

Hymenoscyphus fraxineus is an invasive fungal species causing the most serious disease of ashes (Fraxinus spp.) in Europe—ash dieback. The biology of this fungus is not totally elucidated, neither its relation to the saprophytic species Hymenoscyphus albidus, native in Europe. Our study is focused on the description of seasonal spore dispersal of both fungi and its relation to meteorological conditions, which is needed for more precise and effective control of the disease. For this experiment one long time infected mixed forest in the SE Czech Republic was chosen. A seven-day automatic volumetric spore trap and a weather station were installed to continuously sample the aerospora from April to October 2014. In seven periods a rotating arm spore trap was also used to obtain 48-h air samples to compare the efficiency of these two types of air samplers. Air samples were evaluated solely by qPCR with a very low detection limit. Results show co-occurrence of inoculum of both fungi throughout the entire sampling period with peak levels in August. The origin of the inoculum sampled in the periods without apothecia is discussed. Air-inoculum occurrence of both fungi is significantly correlated with each other, suggesting their coexistence in this forest.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.