TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner

Viruses

Tanja Kahle¹  Bianca Volkmann¹  Kristin Eissmann¹  Alexandra Herrmann¹  Sven Schmitt¹  Sabine Wittmann¹  Laura Merkel¹  Nina Reuter¹  Thomas Stamminger¹  Thomas Gramberg¹ 

[1]Institute of Clinical and Molecular Virology, Friedrich-Alexander University Erlangen-Nürnberg, 91054 Erlangen, Germany

关键词: PML;    TRIM19;    ND10;    HIV-1;    retrovirus;    intrinsic immunity;   

DOI  :  10.3390/v8010002

来源: mdpi

【 摘 要 】

The promyelocytic leukemia protein (PML) is the main structural component of the nuclear matrix structures termed nuclear domain 10 (ND10) or PML nuclear bodies (PML-NBs). PML and ND10 structures have been shown to mediate an intrinsic immune response against a variety of different viruses. Their role during retroviral replication, however, is still controversially discussed. In this study, we analyzed the role of PML and the ND10 components Daxx and Sp100 during retroviral replication in different cell types. Using cell lines exhibiting a shRNA-mediated knockdown, we found that PML, but not Daxx or Sp100, inhibits HIV and other retroviruses in a cell type-dependent manner. The PML-mediated block to retroviral infection was active in primary human fibroblasts and murine embryonic fibroblasts but absent from T cells and myeloid cell lines. Quantitative PCR analysis of HIV cDNA in infected cells revealed that PML restricts infection at the level of reverse transcription. Our findings shed light on the controversial role of PML during retroviral infection and show that PML contributes to the intrinsic restriction of retroviral infections in a cell type-dependent manner.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.