Can Theory Improve the Scope of Quantitative Metazoan Metabarcoding?

Diversity

John P. Wares²  Paula Pappalardo¹ 

[1] Odum School of Ecology, University of Georgia, Athens, GA 30602, USA;;Department of Genetics and Odum School of Ecology, University of Georgia, Athens, GA 30602, USA

关键词: biodiversity;    species diversity;    population genetics;    summary statistics;    coalescent theory;    metabarcoding;   

DOI  :  10.3390/d8010001

来源: mdpi

【 摘 要 】

Using high-throughput sequencing approaches to quantify biodiversity has a number of hurdles, in particular that the number of reads for a given taxon may not be proportional to the number of individuals of that taxon in a sample. Here, we consider whether summary statistics generated in the course of population genetic analyses (such as estimates of haplotype diversity and mutation rate) may be useful in reverse inference of the number of individuals input to an assay. Although our results show that these statistics—combined with the observed number of segregating sites and number of haplotypes in the assay—may be informative, there remain significant concerns about the ability to “metabarcode” a sample and infer relative species abundance.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.