Specific Metabolites in a Phaeodactylum tricornutum Strain Isolated from Western Norwegian Fjord Water

Marine Drugs

Siv Kristin Prestegard²  Svein Rune Erga⁴  Pia Steinr࿌ken⁴  Svein Are Mjøs³  Gjert Knutsen⁴  Jens Rohloff¹ 

[1] Department of Biology, Norwegian University of Science and Technology, N-7491 Trondheim, Norway;;Bergen Marine Biobank, Department of Biology, University of Bergen, Thormøhlensgate 53A/B, N-5020 Bergen, NorwayDepartment of Chemistry, University of Bergen, Allégaten 42, N-5020 Bergen, Norway;Department of Biology, University of Bergen, Thormøhlensgate 53AB, N-5020 Bergen, Norway;

关键词: Phaeodactylum tricornutum;    two-dimensional gel electrophoresis (2DGE);    volatile organic compounds (VOCs);    hormosirene;    ectocarpene;    fatty acids;    eicosapentaenoic acid (EPA);   

DOI  :  10.3390/md14010009

来源: mdpi

【 摘 要 】

We have searched for special characteristics in growth, protein expression, fatty acids and volatile organic compounds (VOCs) in a local Phaeodactylum tricornutum Bohlin strain (Bergen Marine Biobank), by comparing it with a common accession strain (CCAP). Differences in growth and expressed proteins were detected between the BMB strain and the CCAP strain, and the BMB strain reached the highest cell densities under the given growth conditions. Fatty acid (FA) analyses showed highest relative eicosapentaenoic acid (EPA) levels in the exponential phase (25.73% and 28.31%), and highest levels of palmitoleic acid (16:1 n-7) in the stationary phase (46.36% and 43.66%) in the BMB and CCAP strain, respectively. The most striking finding of the VOCs analyses was the relatively high levels of ectocarpene, 6-((1E)-butenyl)-1,4-cycloheptadiene, hormosirene, and desmarestene and structurally related compounds, which were exclusively detected in the BMB strain. Many of the VOCs detected in the CCAP and, in particular, in the BMB strain have been reported as antimicrobial agents. We suggest that the array of pheromones and antimicrobial substances could be part of an allelopathic strategy of the BMB strain, dominated by oval cells, thus reflecting the benthic life stage of this morphological form. These findings show the potential for bioactive metabolites in the BMB strain.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.