Quantitative Proteomic Analysis of the Response to Zinc, Magnesium, and Calcium Deficiency in Specific Cell Types of Arabidopsis Roots

Proteomes

Yoichiro Fukao²  Mami Kobayashi⁵  Sajad Majeed Zargar⁵  Rie Kurata⁵  Risa Fukui¹  Izumi C. Mori³  Yoshiyuki Ogata¹  Ganesh Kumar Agrawal⁴  Sun Tae Kim⁴ 

[1] Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan;;Department of Bioinformatics, Ritsumeikan University, Kusatsu, Shiga 525-8577, JapanInstitute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710-0046, Japan;;id="af1-proteomes-04-00001">1Department of Bioinformatics, Ritsumeikan University, Kusatsu, Shiga 525-8577, JapGraduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0192, Japan;

关键词: FACS;    iTRAQ;    OFFGEL electrophoresis;    root specific cell types;    14-3-3 protein family;    Arabidopsis thaliana;   

DOI  :  10.3390/proteomes4010001

来源: mdpi

【 摘 要 】

The proteome profiles of specific cell types have recently been investigated using techniques such as fluorescence activated cell sorting and laser capture microdissection. However, quantitative proteomic analysis of specific cell types has not yet been performed. In this study, to investigate the response of the proteome to zinc, magnesium, and calcium deficiency in specific cell types of Arabidopsis thaliana roots, we performed isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative proteomics using GFP-expressing protoplasts collected by fluorescence-activated cell sorting. Protoplasts were collected from the pGL2-GFPer and pMGP-GFPer marker lines for epidermis or inner cell lines (pericycle, endodermis, and cortex), respectively. To increase the number of proteins identified, iTRAQ-labeled peptides were separated into 24 fractions by OFFGFEL electrophoresis prior to high-performance liquid chromatography coupled with mass spectrometry analysis. Overall, 1039 and 737 proteins were identified and quantified in the epidermal and inner cell lines, respectively. Interestingly, the expression of many proteins was decreased in the epidermis by mineral deficiency, although a weaker effect was observed in inner cell lines such as the pericycle, endodermis, and cortex. Here, we report for the first time the quantitative proteomics of specific cell types in Arabidopsis roots.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.