Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis

Metabolites

Kazuo Azuma¹  Yoshiko Hirao²  Yoshihiro Hayakawa²  Yusuke Murahata¹  Tomohiro Osaki¹  Takeshi Tsuka¹  Tomohiro Imagawa¹  Yoshiharu Okamoto¹  Norihiko Ito¹ 

[1] Department of Veterinary Clinical Medicine, Tottori University, 4-101 Koyama-minami, Tottori 680-8533, Japan;Analytical and Measuring Instruments Division, Shimadzu Corporation, 1, Nishinokyo Kuwabaracho, Nakagyo-ku, Kyoto 604-8511, Japan;

关键词: Plasma-free amino acid;    pre-column labeling;    liquid chromatography measurements;    canine plasma amino acid levels;    pre- and post-food intake;   

DOI  :  10.3390/metabo6010003

来源: mdpi

【 摘 要 】

Plasma-free amino acid (PFAA) levels are a useful metric for diagnosing cancer and providing a prognosis. However, the use of analysis of PFAA levels has been limited in the veterinary medicine field. We addressed the application of liquid chromatography (LC) using a pre-column labeling technique for analysis of canine PFAA levels. This method significantly shortened the analysis time relative to conventional methods. No diurnal fluctuations were detected at 9:00 AM in most PFAA levels, and food intake increased the levels of some PFAAs, including valine, leucine, tyrosine, phenylalanine, and proline. These results indicate that LC with pre-column labeling is useful for measuring canine PFAA levels, for which time of day and interval after food intake must be taken into consideration.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.