Asymmetric Mach–Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection

Biosensors

Tatevik Chalyan¹  Romain Guider¹  Laura Pasquardini²  Manuela Zanetti²  Floris Falke³  Erik Schreuder³  Rene G. Heideman³  Cecilia Pederzolli²  Lorenzo Pavesi¹ 

[1]Nanoscience Laboratory, Department of Physics, University of Trento, Via Sommarive 14, 38123 Povo (TN), Italy

[2]LaBSSAH, Fondazione Bruno Kessler, Via Sommarive 18, 38123 Povo, Italy

[3]LioniX B.V., Enschede 7522, The Netherlands

关键词: Mach–Zehnder interferometer;    biosensor;    Aflatoxin M1;    limit of detection;    Fab′;   

DOI  :  10.3390/bios6010001

来源: mdpi

【 摘 要 】

In this work, we present a study of Aflatoxin M1 detection by photonic biosensors based on Si₃N₄ Asymmetric Mach–Zehnder Interferometer (aMZI) functionalized with antibodies fragments (Fab′). We measured a best volumetric sensitivity of 10⁴ rad/RIU, leading to a Limit of Detection below 5 × 10⁻⁷ RIU. On sensors functionalized with Fab′, we performed specific and non-specific sensing measurements at various toxin concentrations. Reproducibility of the measurements and re-usability of the sensor were also investigated.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.