DNA Methylation Analysis: Choosing the Right Method

Biology

Sergey Kurdyukov²  Martyn Bullock¹ 

[1]Cancer Genetics Laboratory, Kolling Institute of Medical Research, University of Sydney, Sydney 2065, Australia

[2]

[3]Genomics Core facility, Kolling Institute of Medical Research, University of Sydney, Sydney 2065, Australia

关键词: DNA methylation;    5-methylcytosine;    CpG islands;    epigenetics;    next generation sequencing;   

DOI  :  10.3390/biology5010003

来源: mdpi

【 摘 要 】

In the burgeoning field of epigenetics, there are several methods available to determine the methylation status of DNA samples. However, choosing the method that is best suited to answering a particular biological question still proves to be a difficult task. This review aims to provide biologists, particularly those new to the field of epigenetics, with a simple algorithm to help guide them in the selection of the most appropriate assay to meet their research needs. First of all, we have separated all methods into two categories: those that are used for: (1) the discovery of unknown epigenetic changes; and (2) the assessment of DNA methylation within particular regulatory regions/genes of interest. The techniques are then scrutinized and ranked according to their robustness, high throughput capabilities and cost. This review includes the majority of methods available to date, but with a particular focus on commercially available kits or other simple and straightforward solutions that have proven to be useful.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.