Direct Identification of Enteroviruses in Cerebrospinal Fluid of Patients with Suspected Meningitis by Nested PCR Amplification

Viruses

Alexandr Krasota¹  Natalia Loginovskih²  Olga Ivanova³  Galina Lipskaya¹ 

[1] Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119899, Russia;Hygienic and Epidemiological Center in the Omsk Region, Omsk 644116, Russia;Chumakov Institute of Poliomyelitis and Viral Encephalitides, Moscow 142782, Russia;

关键词: Enterovirus;    nested PCR;    cerebrospinal fluids (CSF);    direct genotyping;    detection;    identification;    sequencing;   

DOI  :  10.3390/v8010010

来源: mdpi

【 摘 要 】

Enteroviruses, the most common human viral pathogens worldwide, have been associated with serous meningitis, encephalitis, syndrome of acute flaccid paralysis, myocarditis and the onset of diabetes type 1. In the future, the rapid identification of the etiological agent would allow to adjust the therapy promptly and thereby improve the course of the disease and prognosis. We developed RT-nested PCR amplification of the genomic region coding viral structural protein VP1 for direct identification of enteroviruses in clinical specimens and compared it with the existing analogs. One-hundred-fifty-nine cerebrospinal fluids (CSF) from patients with suspected meningitis were studied. The amplification of VP1 genomic region using the new method was achieved for 86 (54.1%) patients compared with 75 (47.2%), 53 (33.3%) and 31 (19.5%) achieved with previously published methods. We identified 11 serotypes of the Enterovirus species B in 2012, including relatively rare echovirus 14 (E-14), E-15 and E-32, and eight serotypes of species B and 5 enteroviruses A71 (EV-A71) in 2013. The developed method can be useful for direct identification of enteroviruses in clinical material with the low virus loads such as CSF.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.