Repair of the Orbital Wall Fractures in Rabbit Animal Model Using Nanostructured Hydroxyapatite-Based Implant

Nanomaterials

Sinziana Gradinaru⁴  Laura Madalina Popescu²  Roxana Mioara Piticescu²  Sabina Zurac¹  Radu Ciuluvica³  Alexandrina Burlacu⁵  Raluca Tutuianu⁵  Sorina-Nicoleta Valsan²  Adrian Mihail Motoc²  Liliana Mary Voinea⁴ 

[1] Pathology Department, “Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, RomaniaNational R & D Institute for Non-ferrous and Rare Metals, 077145 Ilfov, Romania;Anatomy Department, “Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, Romania;Ophthalmology Department, “Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, Romania;Institute of Cellular Biology and Pathology “Nicolae Simionescu”, 050568 Bucharest, Romania;

关键词: nanostructured HAp;    mesenchymal stem cells;    orbital wall fracture;    implant;   

DOI  :  10.3390/nano6010011

来源: mdpi

【 摘 要 】

Cellular uptake and cytotoxicity of nanostructured hydroxyapatite (nanoHAp) are dependent on its physical parameters. Therefore, an understanding of both surface chemistry and morphology of nanoHAp is needed in order to be able to anticipate its in vivo behavior. The aim of this paper is to characterize an engineered nanoHAp in terms of physico-chemical properties, biocompatibility, and its capability to reconstitute the orbital wall fractures in rabbits. NanoHAp was synthesized using a high pressure hydrothermal method and characterized by physico-chemical, structural, morphological, and optical techniques. X-ray diffraction revealed HAp crystallites of 21 nm, while Scanning Electron Microscopy (SEM) images showed spherical shapes of HAp powder. Mean particle size of HAp measured by DLS technique was 146.3 nm. Biocompatibility was estimated by the effect of HAp powder on the adhesion and proliferation of mesenchymal stem cells (MSC) in culture. The results showed that cell proliferation on powder-coated slides was between 73.4% and 98.3% of control cells (cells grown in normal culture conditions). Computed tomography analysis of the preformed nanoHAp implanted in orbital wall fractures, performed at one and two months postoperative, demonstrated the integration of the implants in the bones. In conclusion, our engineered nanoHAp is stable, biocompatible, and may be safely considered for reconstruction of orbital wall fractures.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.