Preparation of Extracellular Matrix Developed Using Porcine Articular Cartilage and In Vitro Feasibility Study of Porcine Articular Cartilage as an Anti-Adhesive Film

Materials

Ji Hye Baek²  Kyungsook Kim²  Soon Sim Yang²  Seung Hun Park²  Bo Ram Song²  Hee-Woong Yun²  Sung In Jeong¹  Young Jick Kim³  Byoung Hyun Min²  Moon Suk Kim² 

[1] Advanced Radiation Technology, Korea Atomic Energy Research Institute, Jeollabuk-do 580-185, Korea;Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea;Cell Therapy Center, Ajou University Medical Center, Suwon 443-749, Korea;

关键词: porcine articular cartilage;    anti-adhesive film;    cross-linking;    thermal treatment;    incubation;   

DOI  :  10.3390/ma9010049

来源: mdpi

【 摘 要 】

In this study, we examined whether porcine articular cartilage (PAC) is a suitable and effective anti-adhesive material. PAC, which contained no non-collagenous tissue components, was collected by mechanical manipulation and decellularization of porcine knee cartilage. The PAC film for use as an anti-adhesive barrier was easily shaped into various sizes using homemade silicone molds. The PAC film was cross-linked to study the usefulness of the anti-adhesive barrier shape. The cross-linked PAC (Cx-PAC) film showed more stable physical properties over extended periods compared to uncross-linked PAC (UnCx-PAC) film. To control the mechanical properties, Cx-PAC film was thermally treated at 45 °C or 65 °C followed by incubation at room temperature. The Cx-PAC films exhibited varying enthalpies, ultimate tensile strength values, and contact angles before and after thermal treatment and after incubation at room temperature. Next, to examine the anti-adhesive properties, human umbilical vein endothelial cells (HUVECs) were cultured on Cx-PAC and thermal-treated Cx-PAC films. Scanning electron microscopy, fluorescence, and MTT assays showed that HUVECs were well adhered to the surface of the plate and proliferated, indicating no inhibition of the attachment and proliferation of HUVECs. In contrast, Cx-PAC and thermal-treated Cx-PAC exhibited little and/or no cell attachment and proliferation because of the inhibition effect on HUVECs. In conclusion, we successfully developed a Cx-PAC film with controllable mechanical properties that can be used as an anti-adhesive barrier.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.