Comparative Proteomic Analysis of Mature and Immature Oocytes of the Swamp Buffalo (Bubalus bubalis)

International Journal of Molecular Sciences

Qiang Fu³  Zhen-Fang Liu¹  Yu-Lin Huang³  Yang-Qing Lu³  Ming Zhang³  David Sheehan² 

[1] Reproductive Medicine Center, Xiamen 361000, Fujian, China;;State Key Laboratory of Subtropical Agro-Bioresource Conservation and Utilization, Guangxi University, Nanning 530004, Guangxi, ChinaState Key Laboratory of Subtropical Agro-Bioresource Conservation and Utilization, Guangxi University, Nanning 530004, Guangxi, China;

关键词: swamp buffalo;    oocyte;    comparative proteomics;    heat shock protein 60 (HSP60);    Gem-associated Protein 8 (GEMIN8);    Western blotting;   

DOI  :  10.3390/ijms17010094

来源: mdpi

【 摘 要 】

Maternal protein components change markedly during mammalian oogenesis. Many of these proteins have yet to be characterized and verified. In this study, a proteomics approach was used to evaluate changes in proteins during oogenesis in the Swamp Buffalo (Bubalus bubalis). Proteins from 500 immature oocytes and 500 in vitro matured oocytes were subjected to two-dimensional electrophoresis, and more than 400 spots were detected. Image analysis indicated that 17 proteins were differentially expressed between the two groups. Eight proteins were identified by mass spectrometry. In mature oocytes, three proteins were down-regulated: major vault protein (MVP), N-acetyllactosaminide β-1,6-N-acetylglucosaminyl-transferase (GCNT-2), and gem-associated protein (GEMIN)8, whereas five other proteins, heat shock protein (HSP)60, Ras-responsive element-binding protein 1 (RREB-1), heat shock cognate 71 kDa protein (HSC71), hemoglobin subunit α (HBA), and BMP-2-inducible protein kinase (BMP-2K), were up-regulated. The expression profiles of HSP60 and GEMIN8 were further verified by Western blotting. The changes in HSP60 protein expression demonstrate the increasing need for mitochondrial protein importation to facilitate macromolecular assembly during oocyte maturation. The down-regulation of GEMIN8 production implies that RNA splicing is impaired in mature oocytes.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.