The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr

Molecules

Magdalena Wójciak-Kosior⁵  Ireneusz Sowa⁵  Tomasz Blicharski³  Maciej Strzemski⁵  Sᐪwomir Dresler²  Grażyna Szymczak⁴  Artur Wnorowski¹  Ryszard Kocjan⁵  Ryszard Świeboda⁶ 

[1] Department of Biopharmacy, Medical University of Lublin, Chodźki 4a, Lublin 20-093, Poland;Department of Plant Physiology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Akademicka 19, Lublin 20-033, Poland;Orthopaedics and Rehabilitation Clinic, Medical University Lublin, Chodźki 4a, Lublin 20-093, Poland;Botanical Garden of Maria Curie-Skłodowska University in Lublin, Sławinkowska 3, Lublin 20-810, Poland;Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, Lublin 20-093, Poland;Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4a, Lublin 20-093, Poland;

关键词: soy;    Glycine max;    phytoestrogens;    isoflavones;    coumestrol;    strontium.;   

DOI  :  10.3390/molecules21010090

来源: mdpi

【 摘 要 】

The amount of secondary metabolites in plants can be enhanced or reduced by various external factors. In this study, the effect of strontium ions on the production of phytoestrogens in soybeans was investigated. The plants were treated with Hoagland’s solution, modified with Sr²⁺ with concentrations ranging from 0.5 to 3.0 mM, and were grown for 14 days in hydroponic cultivation. After harvest, soybean plants were separated into roots and shoots, dried, and pulverized. The plant material was extracted with methanol and hydrolyzed. Phytoestrogens were quantified by HPLC. The significant increase in the concentration of the compounds of interest was observed for all tested concentrations of strontium ions when compared to control. Sr²⁺ at a concentration of 2 mM was the strongest elicitor, and the amount of phytoestrogens in plant increased ca. 2.70, 1.92, 3.77 and 2.88-fold, for daidzein, coumestrol, genistein and formononetin, respectively. Moreover, no cytotoxic effects were observed in HepG2 liver cell models after treatment with extracts from 2 mM Sr²⁺-stressed soybean plants when compared to extracts from non-stressed plants. Our results indicate that the addition of strontium ions to the culture media may be used to functionalize soybean plants with enhanced phytoestrogen content.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.