Using Molecular Networking for Microbial Secondary Metabolite Bioprospecting

Metabolites

Kevin Purves³  Lynsey Macintyre²  Debra Brennan³  Guðmundur Ó. Hreggviðsson¹  Eva Kuttner¹  Margrét E. Ásgeirsdóttir¹  Louise C. Young²  David H. Green³  Ruangelie Edrada-Ebel²  Katherine R. Duncan³ 

[1] Matis, Vinlandsleið 12, Reykjavik 113, Iceland;Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G1 1XQ, UK;Scottish Association for Marine Science, Scottish Marine Institute, Oban PA37 1QA, UK;

关键词: molecular networking;    secondary metabolites;    bioprospecting;    bacteria;    Antarctica;   

DOI  :  10.3390/metabo6010002

来源: mdpi

【 摘 要 】

The oceans represent an understudied resource for the isolation of bacteria with the potential to produce novel secondary metabolites. In particular, actinomyces are well known to produce chemically diverse metabolites with a wide range of biological activities. This study characterised spore-forming bacteria from both Scottish and Antarctic sediments to assess the influence of isolation location on secondary metabolite production. Due to the selective isolation method used, all 85 isolates belonged to the phyla Firmicutes and Actinobacteria, with the majority of isolates belonging to the genera Bacillus and Streptomyces. Based on morphology, thirty-eight isolates were chosen for chemical investigation. Molecular networking based on chemical profiles (HR-MS/MS) of fermentation extracts was used to compare complex metabolite extracts. The results revealed 40% and 42% of parent ions were produced by Antarctic and Scottish isolated bacteria, respectively, and only 8% of networked metabolites were shared between these locations, implying a high degree of biogeographic influence upon secondary metabolite production. The resulting molecular network contained over 3500 parent ions with a mass range of m/z 149–2558 illustrating the wealth of metabolites produced. Furthermore, seven fermentation extracts showed bioactivity against epithelial colon adenocarcinoma cells, demonstrating the potential for the discovery of novel bioactive compounds from these understudied locations.

【 授权许可】

CC BY   
© 2016 by the authors; licensee MDPI, Basel, Switzerland.