Biochemistry Research International | |
Phenolic Extract fromMoringa oleiferaLeaves Inhibits Key Enzymes Linked to Erectile Dysfunction and Oxidative Stress in Rats’ Penile Tissues | |
GaniyuOboh1  Margareth L.Athayde1  Aline A.Boligon1  Adedayo O.Ademiluyi1  Ayokunle O.Ademosun1  Sunday I.Oyeleye1  Tosin A.Olasehinde1  | |
DOI : 10.1155/2015/175950 | |
学科分类:生物化学/生物物理 | |
来源: Hindawi Publishing Corporation | |
【 摘 要 】
This study was designed to determine the antioxidant properties and inhibitory effects of extract fromMoringa oleiferaleaves on angiotensin-I-converting enzyme (ACE) and arginase activitiesin vitro. The extract was prepared and phenolic (total phenols and flavonoid) contents, radical (nitric oxide (NO), hydroxyl (OH)) scavenging abilities, and Fe2+-chelating ability were assessed. Characterization of the phenolic constituents was done via high performance liquid chromatography-diode array detection (HPLC-DAD) analysis. Furthermore, the effects of the extract on Fe2+-induced MDA production in rats’ penile tissue homogenate as well as its action on ACE and arginase activities were also determined. The extract scavengedNO∗,OH∗, chelated Fe2+, and inhibited MDA production in a dose-dependent pattern with IC50values of 1.36, 0.52, and 0.38 mg/mL and 194.23 µg/mL, respectively. Gallic acid, chlorogenic acid, quercetin, and kaempferol were the most abundant phenolic compounds identified in the leaf extract. The extract also inhibited ACE and arginase activities in a dose-dependent pattern and their IC50values were 303.03 and 159.59 µg/mL, respectively. The phenolic contents, inhibition of ACE, arginase, and Fe2+-induced MDA production, and radical (OH∗,NO∗) scavenging and Fe2+-chelating abilities could be some of the possible mechanisms by whichM. oleiferaleaves could be used in the treatment and/or management of erectile dysfunction.
【 授权许可】
Unknown
【 预 览 】
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RO201912140927276ZK.pdf | 1579KB | download |