American Journal of Cancer Research | |
SUMO modification of menin | |
Zi-Jie Feng1  Guang-Hui Jin1  Xiao-Lu Yang1  Buddha Gurung1  Xian-Xin Hua1  | |
关键词: Menin; SUMOylation; SUMO1; K591R; | |
DOI : | |
学科分类:肿瘤学 | |
来源: e-Century Publishing Corporation | |
【 摘 要 】
Menin acts as contextual a tumor suppressor and a tumor promoter, partly via epigenetic regulation of gene transcription. While menin is phosphorylated, it remains unclear whether wild type menin has other post-translational modifications. Here, we report that menin is SUMOylated by SUMO1 in vivo and in vitro, and the SUMOylation is reduced by a SUMO protease. Lysine 591 of menin was covalently modified by SUMO1 and K591R mutation in menin blocked SUMOylation of the C-terminal part of menin in transfected cells. Full-length menin with K591 mutation was still SUMOylated in vivo, suggesting the existence of multiple SUMOylation sites. Menin K591R mutant or menin-SUMO fusion protein still retains the ability to regulate cell proliferation and the expression of the examined menin target genes.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912140867382ZK.pdf | 870KB | download |