期刊论文详细信息
International Journal of Clinical and Experimental Pathology | |
Interaction between fatty acid synthase and human epidermal growth receptor 2 (HER2) in osteosarcoma cells | |
Zhi Li Liu1  Rong Ping Zhou1  Kai Zhao1  Guo Mei Zhang1  Yang Zhou1  Zhi Hong Zhang1  Xin Hua Long1  | |
关键词: Osteosarcoma; FASN; HER2; positive feedback regulation; | |
DOI : | |
学科分类:生理学与病理学 | |
来源: e-Century Publishing Corporation | |
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【 摘 要 】
Elevated expression of fatty acid synthase (FASN) and human epidermal growth factor receptor-2 (HER2) are observed in human osteosarcoma (OS). The aim in this study is to investigate a possible connection between FASN expression and the activity of HER2. The immunohistochemistry staining was conducted on 24 OS specimens from patients, which revealed a significant positive correlation between FASN and HER2 as well as p-HER2 protein expression. Furthermore, the human OS cell lines MG-63 and U2-OS were treated with FASN-specific RNAi Plasmid or Lapatinib (an inhibitor of HER2). The mRNA of HER2 and FASN was measured using RT-PCR. Western blot was performed to detect the protein expression of HER2, p-HER2 and FASN. The results demonstrated that HER2 modulates FASN expression, inhibition of FASN resulted in down-regulation of HER2 and p-HER2 protein in OS cells. Our findings suggested that there was positive feedback regulation between FASN and HER2 expression and phosphorylation in OS cells.【 授权许可】
Unknown
【 预 览 】
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RO201912140867076ZK.pdf | 856KB | ![]() |