Up-regulation of ECT2 is associated with poor prognosisn gastric cancer patients

International Journal of Clinical and Experimental Pathology

Haibo Yao¹  Yuhui Yu¹  Ruxuan Zhang¹  Yi Jin¹  Yingyu Ma¹  Qinshu Shao¹  Yuan Xu¹ 

关键词: ECT2;    microarray;    gastric cancer;    prognosis;   

DOI  :  

学科分类：生理学与病理学

来源: e-Century Publishing Corporation

【 摘 要 】

Objectives: The aim of this study was to investigate the expression of ECT2 in gastric cancer and its clinical significance. Methods and results: We investigated the differentially expressed genes between gastric cancer tissues and normal gastric mucosa by cDNA microarray, and then we found ECT2 was up-regulated in gastric cancer. What is more, we verified ECT2 expression level by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and measured its protein level by immunohistochemistry (IHC). qRT-PCR analysis indicated ECT2 was significantly up-regulated in gastric cancer and Immunohistochemistry confirmed the percentage of ECT2-positive specimens was significantly higher in gastric carcinoma than in non-tumor tissues. Up-regulation of ECT2 is associated with the degree of histological differentiation (P = 0.007), invasion depth (P = 0.047), lymph node metastasis (P = 0.016), distant metastasis (P = 0.021) and TNM stage (P = 0.016), patients with up-regulated ECT2 had a lower overall survival rate (P = 0.000). Cox regression analysis revealed that up-regulation of ECT2 is an independent prognostic factor in gastric cancer patients (P = 0.012). Conclusion: Up-regulation of ECT2 might contribute to the progression of gastric carcinogenesis and may be a useful prognostic indicator in gastric cancer.

【 授权许可】

Unknown