HPV frequency in penile carcinoma of Mexican patients: important contribution of HPV16 European variant

International Journal of Clinical and Experimental Pathology

AnaE Romero¹  Brenda Alatorre¹  Beatriz González-Yebra¹  Patricia Piña-Sánchez¹  Ricardo López-Romero¹  Karla Vázquez¹  Candela Iglesias-Chiesa¹  Isabel Alvarado¹  Raúl Peralta¹  Minerva Lazos¹  Mauricio Salcedo¹ 

关键词: HPV;    penile carcinoma;    México;   

DOI  :  

学科分类：生理学与病理学

来源: e-Century Publishing Corporation

【 摘 要 】

The role of human papillomavirus (HPV) infection in penile carcinoma (PeC) is currently reported and about half of the PeC is associated with HPV16 and 18. We used a PCR-based strategy by using HPV general primers to analyze 86 penile carcinomas paraffin-embedded tissues. Some clinical data, the histological subtype, growth pattern, and differentiation degree were also collected. The amplified fragments were then sequenced to confirm the HPV type and for HPV16/18 variants. DNA samples were also subjected to relative real time PCR for hTERC gene copy number. Some clinical data were also collected. Global HPV frequency was 77.9%. Relative contributions was for HPV16 (85%), 31 (4.4%), 11 (4.4%), 58, 33, 18, and 59 (1.4% each one). Sequence analysis of HPV16 identified European variants and Asian-American (AAb-c) variants in 92% and in 8% of the samples, respectively. Furthermore hTERC gene amplification was observed in only 17% of the cases. Our results suggest that some members of HPV A9 group (represented by HPV16, 58, and 31) are the most frequent among PeC patients studied with an important contribution from HPV16 European variant. The hTERC gene amplification could be poorly related to penile epithelial tissue.

【 授权许可】

Unknown