Green Tea-EGCG reduces GFAP associated neuronal loss in HIV-1 Tat transgenic mice

American Journal of Translational Research

Jun Tian¹  Francisco Fernandez¹  Brian Giunta¹  Elona Rrapo¹  Huayan Hou¹  Jun Tan¹  Adam Smith¹  Yuyan Zhu¹ 

关键词: HIV;    Tat;    dementia;    astrocytosis;    green tea;    (–)-epigallocatechin-3-gallate (EGCG);   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

In the current era of antiretroviral treatment, the prevalence of HIV-associated dementia is on the rise. Many past works have associated inflammation and neuronal loss with cognitive deficits inherent to the syndrome. Importantly, HIV-1 induced astrogliosis has been shown to play a central role in this process. Here we examined the effect of green tea derived (–)-epigallocatechin-3-gallate (EGCG) food supplementation for its ability to modulate GFAP expression and neuronal loss in an HIV-1 Tat transgenic mouse model whose expression was controlled by a brain specific doxycycline promoter. By immunohistochemistry we found that EGCG (300mg/kg/day) dramatically reduced astrogliosis as demonstrated by GFAP expression. This was accompanied by a mild reduction in activated microglia by Iba-1 staining and significant reduction in neuronal loss through apoptosis as demonstrated by MAP2 staining and Western blot analysis respectively. Future studies will be required to determine intracellular mechanism involved in EGCG mediated downregulation of GFAP and associated astrocytosis and neuronal loss.

【 授权许可】

Unknown