Molecular classification and drug resistance analysis of Escherichia coli isolated from poultry in China

International Journal of Clinical and Experimental Medicine

Jinghua Cui¹  Juan Wang¹  Junwei Wang¹  Xiumei Huang¹  Jianmei Zhao¹  Zhigang Cui¹  Wenyan Gai¹  Zhina Qu¹  Xiaoli Du¹ 

关键词: Escherichia coli;    virulence genes;    resistance gene;    drug resistance;    pulsed-field gel electrophoresis;    multilocus sequence analysis;    poultry;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

We aimed to understand the distribution of Escherichia coli in poultry and to reveal the virulence factors, the drug resistance and molecular epidemic regularity and characteristics of isolate strains from 6 provinces in China and to complete the characteristics of E. coli for the risk assessment. A total of 87 E. coli isolates were analyzed with 7 virulence genes by PCR drug sensitivity test in 13 kinds of antimicrobial agents and analyzed with PFGE and MLST genotyping. The PFGE genotyping of 87 isolates yielded 75 PFGE type. MLST analysis of isolates identified the 39 STs, the 7 housekeeping genes had the different variation. The most prevalent virulence genes were iucD (74.7%), followed by iss (55.2%), Irp2 (43.7%), tsh (28.7%), cva (19.5%), papC (9.2%) and vat (8.1%). All isolates were resistant to two or three antimicrobial agents highly resistant to SXT, TE (85.06%), SF (83.91%), AM (66. 67%), to fluoroquinolones (ENR, 63.22%, NOR, 50.57%) and to GM (57.47%). E. coli strains resistant spectrum was wide gene was polymorphism the distribution had a certain timeliness and regional in part region of China. These were a solid foundation for the epidemiological investigation and traceability laid.

【 授权许可】

Unknown