Biological characteristics of CD133+ cancer stem cells derived from human laryngeal carcinoma cell line

International Journal of Clinical and Experimental Medicine

Jing Chen¹  Jian He¹  Xudong Wei¹  Bingjuan Ma¹  Jingyu Wang¹ 

关键词: CD133;    laryngeal carcinoma;    tumor stem cells;    chemotherapy;    radiotherapy;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Objective: To investigate the in vitro invasive capability, clone-forming ability, resistance to anti-tumor treatments of CD133⁺ human laryngeal carcinoma stem cells, and characterize the related signaling pathways in these cells. Methods: Human laryngeal carcinoma Hep-2 cells were subjected to flow cytometry sorting to obtain CD133⁺ stem cells. Transwell chamber assay and clone-formation forming test were performed to evaluate the invasive capability and the clone-forming ability of CD133⁺ laryngeal carcinoma tumor stem cells, respectively. MTT assay was used to assess the resistance of CD133⁺ Hep-2 cells to radiotherapy and chemotherapy, respectively. Western blot and real-time PCR were applied to characterize the signaling pathways in these stem cells. Results: Our results from the transwell chamber assay indicated that the migrating capability of CD133⁺ Hep-2 cells was significantly higher than CD133^- cells, and the invasive capability of CD133⁺ Hep-2 cells was also significantly elevated. Moreover, clone-formation forming test showed higher clone-forming ability for CD133⁺ Hep-2 cells, compared with CD133^- cells. Furthermore, CD133⁺ Hep-2 cells displayed significant resistance to radiotherapy and chemotherapy. The Bcl-2/Bax ratio was increased, and Hedgehog, Wnt, and Bmi-l signaling pathways were all activated, in CD133⁺ laryngeal carcinoma stem cells, which might be involved in the self-renewal process of these stem cells. Conclusion: The invasive capability, clone-forming ability, and resistance to anti-tumor treatments are enhanced, and anti-apoptotic and proliferation-related signaling pathways are activated in CD133⁺ laryngeal carcinoma tumor stem cells. These findings might provide new insights into the prevention and/or treatment of laryngeal carcinoma, especially concerning target-oriented therapies.

【 授权许可】

Unknown