Serine threonine kinase Pim-3 regulates STAT3 pathway to inhibit proliferation of human liver cancers

International Journal of Clinical and Experimental Medicine

Yuan Huang¹  Jianqiang Wang¹  Lijun Lao¹  Hui Zhao¹ 

关键词: Serine threonine kinase Pim-3;    liver tumor;    STAT3;    apoptosis;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Objective: This study aimed to investigate the effects of serine threonine kinase Pim-3 on the growth of HepG2 cells and to explore the role of STAT3 signaling pathway. Methods: Synthetic Pim-3shRNA and negative control shRNA were independently transfected into HepG2 cells in the presence of Lipofectamine^TM 2000. Cells were divided into 4 groups: Pim-3 shRNA group, negative control group, liposome control group, and blank control group. Flow cytometry was performed to detect the apoptosis of these cells; RT-PCR was employed to detect the mRNA expression of Pim-3; Western blot assay was done to measure the protein expression of Pim-3, STAT3, pSTAT3^Tyr705, Bcl-Xl, Bad and pBad^Ser112. Results: When compared with blank control group, liposome group and negative control group, the apoptosis index increased and the protein expression of Pim-3, pSTAT3^Tyr705, Bcl-Xl and pBad^Ser112 and the Pim-3 mRNA expression reduced in the Pim-3 shRNA group, but the protein expression of STAT3 and Bad was comparable among groups. Conclusion: Pim-3 shRNA may down-regulate pSTAT3^Tyr705 and pBad^Ser112 protein expression to inhibit the proliferation of liver cancer cells and Pim-3 may serve as a target for the treatment of liver cancer.

【 授权许可】

Unknown