Electrophysiology properties of voltage-gated potassium channels in rat peritoneal macrophages

International Journal of Clinical and Experimental Medicine

Er-Bao Bian¹  Xiao-Hua Wang¹  Jun Li¹  Huang Yan¹  Huang Cheng¹  Xiong-Wen Lv¹  Bao-Ming Wu¹  Bin Zhao¹  Zhi-Gang Xiong¹ 

关键词: Patch-clamp;    peritoneal macrophage;    potassium channel;    TEA;    ATP;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Ion channels are important for the functions of excitable and non-excitable cells. Using the whole-cell patch clamp technique, we analyzed the electrophysiological and pharmacological properties of voltage-gated potassium channels in primary rat peritoneal macrophages. With intracellular solution contained K⁺ as the main charge carrier, all cells showed outward currents in response to membrane depolarization. The currents can be inhibited by TEA (10 mM), a non-selective blocker for voltage-gated K⁺ channels, and attenuated when intracellular K⁺ was substituted with Cs⁺. Changing holding potential from -80 to -30 mV or -10 mV also inhibited the outward currents. In contrast, increasing the concentration of ATP in the intracellular solution decreased the amplitude of the outward currents. Thus, rat peritoneal macrophages express several types of functional voltage-gated K⁺ channels.

【 授权许可】

Unknown