Acid stress response of a mycobacterial proteome: insight from a gene ontology analysis

International Journal of Clinical and Experimental Medicine

Bryan AP Roxas¹  Qingbo Li¹ 

关键词: Mycobacterium smegmatis;    acid stress;    label-free proteomics;    gene ontology;    systems biology;    membrane transport;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Acidity in vesicles of macrophages is a general signal that bacteria respond to during infection. Mycobacteria are particularly capable of resisting the acidification in macrophages that engulf the bacteria. In this work, we used label-free quantitative proteomics to study the Mycobacterium smegmatis proteome under acid stress so as to gain an insight into the acidic adaptation in mycobacteria. We quantified 1032 proteins. With a 3-fold change threshold, 20 and 52 proteins were found regulated at false discovery rates of 5% and 14% respectively. We performed a systems analysis based on gene ontology for the global proteome expression profile. We found that the most significant changes induced by the acid stress include a downregulation of transmembrane transporter activity and an upregulation of enzymes involved in fatty acid metabolism. The results suggest that reduced transmembrane transport and increased fatty acid metabolism probably contribute to or associate with acid tolerance in mycobacteria.

【 授权许可】

Unknown