Correlations between total cell concentration, total adenosine tri-phosphate concentration and heterotrophic plate counts during microbial monitoring of drinking water

Drinking Water Engineering and Science

Y.Wang¹  F.Hammes¹  T.Egli¹  E.Siebel¹ 

DOI  :  10.5194/dwes-1-1-2008

学科分类：工程和技术（综合）

来源: Copernicus Publications

【 摘 要 】

The general microbial quality of drinking water is normally monitored byheterotrophic plate counts (HPC). This method has been used for more than100 years and is recommended in drinking water guidelines. However, the HPCmethod is handicapped because it is time-consuming and restricted toculturable bacteria. Recently, rapid and accurate detection methods haveemerged, such as adenosine tri-phosphate (ATP) measurements to assessmicrobial activity in drinking water, and flow cytometry (FCM) to determinethe total cell concentration (TCC). It is necessary and important fordrinking water quality control to understand the relationships among theconventional and new methods. In the current study, all three methods wereapplied to 200 drinking water samples obtained from two local buildingsconnected to the same distribution system. Samples were taken both on normalworking days and weekends, and the correlations between the differentmicrobiological parameters were determined. TCC in the samples ranged from0.37–5.61×10⁵ cells/ml, and two clusters, the so-calledhigh (HNA) and low (LNA) nucleic acid bacterial groups, were clearlydistinguished. The results showed that the rapid determination methods(i.e., FCM and ATP) correlated well (R²=0.69), but only a weakcorrelation (R²=0.31) was observed between the rapid methods andconventional HPC data. With respect to drinking water monitoring, both FCMand ATP measurements were confirmed to be useful and complimentaryparameters for rapid assessing of drinking water microbial quality.

【 授权许可】

Unknown